Reverse transcription polymerase chain reaction

I reverse-transcript RNA to a mold, and a reverse transcription polymerase chain reaction (ぎゃくてんしゃ polymerase れんさはんのう, Reverse Transcription Polymerase Chain Reaction, RT-PCR) is a method to perform PCR for produced cDNA.

I attach a primer to DNA becoming the mold by the PCR method and specifically detect DNA caught in the objective primer sequence by DNA polymerase. I can use the PCR method for the detection of DNA, but cannot detect the RNA. Therefore I convert RNA into cDNA by reverse transcription and perform the PCR method for the cDNA.

For example, some viruses such as retroviruses have only RNA. When I prove the infection of such a virus, I will use the RT-PCR method. MRNA existing in a cell is the material which is very more unstable than DNA, and half-life is said about for half a year even if I cryopreserve it at -80 degrees Celsius. Therefore I may use RT-PCR for the purpose of storing mRNA sequence semipermanently.

Allied item

• Reverse transcriptase
• PCR
• RNA virus

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